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Probeset ID AF203339.1_s_at
Current gene ID AGAP006911 (AGAP006911 is also associated with: AF203339.1_at - Ag.2L.14.0_CDS_at)
Affymetrix transcript data AF203339.1 /DEF=Anopheles gambiae immune-responsive serpin-related protein ISerpF1 (ISerpF1) mRNA, partial cds. /DB_XREF=gi:6635470


STAGE SIGNAL MEAN STD DEV
L 3328.73 552.6
M 4891.83 343.13
NBF 2359.93 163.72
BF3h 5009.63 242.48
BF24h 2788.47 31.19
BF48h 3295.17 324.4
BF72h 2924.63 220.12
BF96h 3721.57 243.69
BF15d 4618.47 86.26
TISSUE SIGNAL MEAN STD DEV
Fat bodies 5713.73 1110.43
Midgut 66.8 55.71
Ovaries 2929.6 237.2


ENSEMBL ANNOTATION
VECTORBASE ANNOTATION
USER-CONTRIBUTED NOTES (0)

AFFYMETRIX ANNOTATION
Probeset ID AF203339.1_s_at
Annotation Date 3/8/2007
Genome Version February 2003 (IAGP v.MOZ2)
Alignments chr2L:39329357-39329810 (+) // 96.79 //
Ensembl ENSANGG00000019323
SwissProt Q9U451
Gene Ontology Molecular Function 0004867 // serine-type endopeptidase inhibitor activity // inferred from electronic annotation
InterPro IPR000215 // Proteinase inhibitor I4, serpin // 2.1E-25
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a Entrez Gene identifier using 1 transcripts. // false // Matching Probes // A
Annotation Transcript Cluster XM_308845(11)
Transcript Assignments ENSANGESTT00000002961 // --- // ensembl_est // 11 // --- /// ENSANGESTT00000002962 // --- // ensembl_est // 11 // --- /// BX019858 // Single read from an extremity of a full-length cDNA clone made from Anopheles gambiae total adult females. 5-PRIME end of
Annotation Notes BX010659 // gb // 8 // Cross Hyb Matching Probes /// BX019859 // gb // 6 // Negative Strand Matching Probes /// BX027147 // gb // 11 // Negative Strand Matching Probes

ANOXCEL ANNOTATION
Seq name AGAP006911-PA
First residue M
Description pep:known chromosome:AgamP3:2L:39896875:39899752:1 gene:AGAP006911 transcript:AGAP006911-RA
CDS name and link to nucleotide data AGAP006911-RA
Description cdna:known chromosome:AgamP3:2L:39896875:39899752:1 gene:AGAP006911
Best match to AG-CDS AGAP006911-RA
E value 0
Score 820
Extent of match 409
Length of best match 1541
% identity 100
% Match length 27
First residue of match - UTR overhang 212
First residue of sequence 1
Link to whole gene + 2000nt 5' + 200nt 3' AGAP006911-PA
Link to 5' 2,000 nt AGAP006911-PA
Link to 3' 200 nt AGAP006911-PA
Gene name and view at Ensembl AGAP006911
Protein view at Ensembl AGAP006911-PA
Chromosome 2L
For or Rev F
Location join(39897086..39897116,39898248..39898345,39898414..39899226,39899294..39899444,39899516..39899652)
Exon locations 39897086-39897116 39898248-39898345 39898414-39899226 39899294-39899444 39899516-39899652
Number of introns and link to fasta file 4
intron lengths 1132 69 68 72
Start 39897086
End 39899652
Gene length 2566
Protein size 409
Complexity index 0.27
Cys number 1
SigP Result SIG
Cleavage Position 21-22
MW 46.557
pI 5.21
Mature MW 44.296
pI 5.2
TMHMM result ExpAA=1.96 First60=1.88 PredHel=0 Topology=o
Predicted helices 0
Best match to NR protein database serpin 2
E value 0.0
Match gi|116041593
Score 817
Extent of match 409
Length of best match 409
% identity 99
% Match length 100
First residue of match 1
First residue of sequence 1
Number of segments 1
Species Anopheles gambiae
Key words SERPIN 2 ANOPHELES GAMBIAE SERINE PROTEASE INHIBITOR AEDES AEGYPTI IMMUNE-RESPONSIVE SERPIN-RELATED ISERPF1 IMMUNE RESPONSIVE RELATED 27A GLOSSINA MORSITANS DROSOPHILA PSEUDOOBSCURA >GI SERPIN-27A MELANOGASTER SALIVARY LUTZOMYIA LONGIPALPIS 1 3 PXSERPIN PLUTELLA XYLOSTELLA BOMBYX MORI 3A MANDUCA SEXTA SIMILAR TO MELLIFERA TRIBOLIUM CASTANEUM HYPHANTRIA CUNEA ANTITHROMBIN III MACACA MULATTA OR CYSTEINE PROTEINASE CLADE C MEMBER MUS MUSCULUS ANT3_MOUSE ANTITHROMBIN-III PRECURSOR ATIII MOUSE AT-III SP. PEPTIDASE UNNAMED PRODUCT ANT3_PONPY HYPOTHETICAL PONGO PYGMAEUS ISOFORM PAN TROGLODYTES RATTUS NORVEGICUS
Best match to SWISSP database Antithrombin-III precursor (ATIII) - Mus musculus (Mouse)
E value 2E-048
Match P32261|ANT3_MOUSE
Score 192
Extent of match 370
Length of best match 465
% identity 32
% Match length 80
First residue of match 103
First residue of sequence 47
Number of segments 1
PROSITE motifs ASN_GLYCOSYLATION |PKC_PHOSPHO_SITE |CK2_PHOSPHO_SITE |MYRISTYL |MICROBODIES_CTER |SERPIN |
Non promiscuous prosite motifs SERPIN |
Best match to GO database serine (or cysteine) peptidase inhibitor, clade C (antithrombin), member 1 - Mus musculus - extracellular space
E value E-4
Function descriptors serine-type endopeptidase inhibitor activity||endopeptidase inhibitor activity||protease inhibitor activity||enzyme inhibitor activity||enzyme regulator activity
Function parent enzyme regulator activity
Function second parent enzyme inhibitor activity
GO # GO:0004867
E value of functional GO 3e-32
Component descriptors cellular_component
Component parent cellular_component
GO # GO:0005575
E value of component GO 3e-32
Process descriptors biological_process
Process parent enzyme regulator activity
Process second parent enzyme inhibitor activity
GO # GO:0008150
E value of process GO 3e-32
Best match to KOG database Serpin
E value 8E-067
General class Defense mechanisms
Best match to PFAM database Serpin
E value 7E-052
Best match to SMART database SERPIN
E value 1E-058
Best match to AEGY-PEP database serine protease inhibit
E value 1e-138
Match AAEL005673-RA
Score 488
Extent of match 409
Length of best match 427
% identity 59
% Match length 96
First residue of match 19
First residue of sequence 1
Number of segments 1
Best match to DMPROT database Spn27A-PA
E value 8E-079
Match Spn27A-PA
Score 290
Extent of match 390
Length of best match 447
% identity 40
% Match length 87
First residue of match 63
First residue of sequence 24
Number of segments 1
Best match to CELEG database srp-2 serine protease inhibitor status:Confirmed TR:O17365 protein_id:AAB71270.1
E value 4E-034
Match C05E4.1
Score 142
Extent of match 351
Length of best match 359
% identity 28
% Match length 98
First residue of match 21
First residue of sequence 49
Number of segments 1
Best match to ARAB database serp
E value 2E-032
Match AT1G47710.1
Score 137
Extent of match 390
Length of best match 391
% identity 26
% Match length 100
First residue of match 10
First residue of sequence 34
Number of segments 1
Best match to YEAST database SW:Q12139 Q12139 saccharomyces cerevisiae (baker's yeast). ypr022c protein.
E value 0.44
Match NR_SC:SW-Q12139
Score 31.2
Extent of match 151
Length of best match 1133
% identity 23
% Match length 13
First residue of match 942
First residue of sequence 37
Number of segments 1
Best match to clusterized An. gambiae ESTs from different libraries all-ests-contig_1529
E value 0
Score 820
Extent of match 409
Length of best match 1424
% identity 100
% Match length 29
First residue of match 89
First residue of sequence 1
Number of segments 1
Orientation of output FOR
Satisfied 95% id on length 100 Y
Number of sequences on assembled contig 58
ESTs coming from from all-instars library 1
Blood-abdomen library 0
Sugar-abd library 0
Infected blood-abd library 0
Big blood fed library 10
Big non blood fed library 4
Head library 5
Immune cells 0
normalized-fatbody 14
Female salivary glands (Ribeiro and Pasteur) 24
Male salivary glands 0
Best match to Affymetrix chip Ag.2L.14.0_CDS_at
E value 0
Extent of match 409
% identity 96
Clustered at 35%-Sim- on 60% of length - - Cluster# 0
# seqs 0
Clustered at 45%-Sim- on 60% of length - - Cluster# 0
# seqs 0
Clustered at 55%-Sim- on 60% of length - - Cluster# 0
# seqs 0
Clustered at 65%-Sim- on 60% of length - - Cluster# 5634
# seqs 1
Clustered at 75%-Sim- on 6% of length - - Cluster# 5783
# seqs 1
Clustered at 85%-Sim- on 60% of length - - Cluster# 5878
# seqs 1
Clustered at 95%-Sim- on 60% of length - - Cluster# 5946
# seqs 1



User Contributed Notes
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AF203339.1_s_at


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