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Probeset ID AY062189.1_s_at
Current gene ID AGAP001076 (AGAP001076 is also associated with: Ag.X.87.0_CDS_a_at)
Affymetrix transcript data AY062189.1 /DEF=Anopheles gambiae cytochrome P450 CYP4G16 (Cyp4G16) mRNA, partial cds. /DB_XREF=gi:18139566


STAGE SIGNAL MEAN STD DEV
L 10852.6 1141.29
M 20368.93 2086.03
NBF 15023.03 910.34
BF3h 6330.27 746.75
BF24h 9600.6 104.35
BF48h 3626.7 237.87
BF72h 3635.4 353.18
BF96h 5778.93 974.24
BF15d 7111.17 875.48
TISSUE SIGNAL MEAN STD DEV
Fat bodies 44256.97 10169.77
Midgut 339.77 267.71
Ovaries 420.8 48.29


ENSEMBL ANNOTATION
VECTORBASE ANNOTATION
USER-CONTRIBUTED NOTES (0)

AFFYMETRIX ANNOTATION
Probeset ID AY062189.1_s_at
Annotation Date 3/8/2007
Genome Version February 2003 (IAGP v.MOZ2)
Alignments chrX:21847624-21848040 (-) // 90.51 //
Ensembl ENSANGG00000014481
SwissProt Q8WQP8
Gene Ontology Biological Process 0006118 // electron transport // inferred from electronic annotation
Gene Ontology Molecular Function 0004497 // monooxygenase activity // inferred from electronic annotation /// 0005506 // iron ion binding // inferred from electronic annotation /// 0016491 // oxidoreductase activity // inferred from electronic annotation /// 0020037 // heme binding // in
InterPro IPR001128 // Cytochrome P450 // 1.4E-52 /// IPR001128 // Cytochrome P450 // 2.7E-53
Trans Membrane ENSANGESTP00000008265 // span:17-39 // numtm:1 /// ENSANGESTP00000008267 // span:17-39 // numtm:1 /// ENSANGESTP00000008271 // span:17-39 // numtm:1 /// XP_558699.3 // span:17-39 // numtm:1
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a Entrez Gene identifier using 1 transcripts. // false // Matching Probes // A
Annotation Transcript Cluster XM_558699(11)
Transcript Assignments ENSANGESTT00000008265 // --- // ensembl_est // 11 // --- /// ENSANGESTT00000008267 // --- // ensembl_est // 11 // --- /// ENSANGESTT00000008271 // --- // ensembl_est // 11 // --- /// AY062189 // Anopheles gambiae cytochrome P450 CYP4G16 (Cyp4G16) mRNA, pa
Annotation Notes ENSANGESTT00000008273 // ensembl_est // 2 // Cross Hyb Matching Probes /// BX065952 // gb // 1 // Cross Hyb Matching Probes /// BX065953 // gb // 2 // Negative Strand Matching Probes

ANOXCEL ANNOTATION
Seq name AGAP001076-PA
First residue M
Description pep:known chromosome:AgamP3:X:22937938:22947129:-1 gene:AGAP001076 transcript:AGAP001076-RA
CDS name and link to nucleotide data AGAP001076-RA
Description cdna:known chromosome:AgamP3:X:22937938:22947129:-1 gene:AGAP001076
Stop? *
Best match to AG-CDS AGAP001076-RA
E value 0
Score 1126
Extent of match 565
Length of best match 2092
% identity 100
% Match length 27
First residue of match - UTR overhang 395
First residue of sequence 1
Link to whole gene + 2000nt 5' + 200nt 3' AGAP001076-PA
Link to 5' 2,000 nt AGAP001076-PA
Link to 3' 200 nt AGAP001076-PA
Gene name and view at Ensembl AGAP001076
Protein view at Ensembl AGAP001076-PA
Chromosome X
For or Rev R
Location join(complement(22946435..22946654),complement(22945133..22945330),complement(22944371..22944553),complement(22938227..22939144),complement(22937938..22938116))
Exon locations 22946435-22946654 22945133-22945330 22944371-22944553 22938227-22939144 22937938-22938116
Number of introns and link to fasta file 4
intron lengths 1105 580 5227 111
Start 22937938
End 22946654
Gene length 8716
Protein size 565
Complexity index 0.21
Cys number 5
SigP Result CYT
MW 64.744
pI 8.83
TMHMM result ExpAA=21.43 First60=21.19 PredHel=1 Topology=i17-39o
Predicted helices 1
% membrane 3.9
% outside 93.1
% inside 3
Best match to NR protein database cytochrome P450
E value 0.0
Match gi|108880360
Score 1013
Extent of match 563
Length of best match 566
% identity 88
% Match length 99
First residue of match 1
First residue of sequence 1
Number of segments 1
Species Aedes aegypti
Key words CYTOCHROME P450 AEDES AEGYPTI SIMILAR TO 4G15 CYPIVG15 TRIBOLIUM CASTANEUM LEPTINOTARSA DECEMLINEATA CYP4G15 ISOFORM A DROSOPHILA MELANOGASTER >GI Q9VYY4 C4G15_DROME PSEUDOOBSCURA CYP4G25 BOMBYX MORI ANTHERAEA YAMAMAI MONOOXYGENASE APIS MELLIFERA ANTENNAL CYP4 MAMESTRA BRASSICAE CYP4G27 PARACONFUSUS CYP4G19 BLATTELLA GERMANICA FAMILY 4 CHIRONOMUS TENTANS 1 2 B CG3972-PA CP4G1_DROME CYPIVG1 CYP4G13V2 MUSCA DOMESTICA CG1438-PA CP4C3_DROME CYPIVC3
Best match to SWISSP database Cytochrome P450 4g15 (EC 1.14.-.-) (CYPIVG15) - Drosophila melanogaster (Fruit
E value 0.0
Match Q9VYY4|C4G15_DROME
Score 816
Extent of match 569
Length of best match 574
% identity 69
% Match length 99
First residue of match 4
First residue of sequence 11
Number of segments 1
PROSITE motifs ASN_GLYCOSYLATION |PKC_PHOSPHO_SITE |CK2_PHOSPHO_SITE |TYR_PHOSPHO_SITE |MYRISTYL |AMIDATION |PRENYLATION |MICROBODIES_CTER |CYTOCHROME_P450 |SUGAR_TRANSPORT_2 |
Non promiscuous prosite motifs CYTOCHROME_P450 |SUGAR_TRANSPORT_2 |
Best match to GO database Cyp4g15 - Drosophila melanogaster - electron carrier activity - microsome - steroid biosynthetic process - membrane
E value
Function descriptors electron carrier activity||oxidoreductase activity||catalytic activity
Function parent catalytic activity
Function second parent oxidoreductase activity
GO # GO:0009055
E value of functional GO 0
Component descriptors microsome||vesicular fraction||membrane fraction||cell fraction
Component parent cell fraction
Component second parent membrane fraction
GO # GO:0005792
E value of component GO 0
Process descriptors steroid biosynthesis||lipid biosynthesis||cellular lipid metabolism||cellular metabolism||cellular physiological process
Process parent catalytic activity
Process second parent oxidoreductase activity
GO # GO:0006694
E value of process GO 0
Best match to KOG database Cytochrome P450 CYP4/CYP19/CYP26 subfamilies
E value 1E-101
General class Secondary metabolites biosynthesis, transport and catabolism, Lipid transport and metabolism
Best match to PFAM database p450
E value 3E-076
Best match to AEGY-PEP database cytochrome P450
E value 0
Match AAEL004054-RA
Score 1013
Extent of match 563
Length of best match 566
% identity 88
% Match length 99
First residue of match 1
First residue of sequence 1
Number of segments 1
Best match to DMPROT database Cyp4g15-PA
E value 0.0
Match Cyp4g15-PA
Score 816
Extent of match 569
Length of best match 574
% identity 69
% Match length 99
First residue of match 4
First residue of sequence 11
Number of segments 1
Best match to CELEG database cyp-42A1 cytochrome P450 status:Partially_confirmed TR:Q9U1R5
E value 1E-081
Match Y80D3A.5
Score 300
Extent of match 515
Length of best match 511
% identity 33
% Match length 101
First residue of match 36
First residue of sequence 55
Number of segments 1
Best match to ARAB database cytochrome P450 family prote
E value 2E-035
Match AT5G38450.1
Score 147
Extent of match 544
Length of best match 518
% identity 23
% Match length 105
First residue of match 28
First residue of sequence 38
Number of segments 1
Best match to YEAST database SW:CP51_YEAST P10614 saccharomyces cerevisiae (baker's yeast). cytochrome p450
E value 9E-016
Match NR_SC:SW-CP51_YEAST
Score 80.5
Extent of match 222
Length of best match 530
% identity 22
% Match length 42
First residue of match 274
First residue of sequence 324
Number of segments 1
Best match to clusterized An. gambiae ESTs from different libraries all-ests-contig_928
E value 0
Score 1080
Extent of match 596
Length of best match 2188
% identity 92
% Match length 27
First residue of match 399
First residue of sequence 1
Number of segments 1
Orientation of output FOR
Number of sequences on assembled contig 50
ESTs coming from from all-instars library 3
Blood-abdomen library 0
Sugar-abd library 0
Infected blood-abd library 0
Big blood fed library 20
Big non blood fed library 18
Head library 0
Immune cells 0
normalized-fatbody 9
Female salivary glands (Ribeiro and Pasteur) 0
Male salivary glands 0
Best match to Affymetrix chip Ag.X.87.0_CDS_a_at
E value 0
Extent of match 565
% identity 97
Clustered at 35%-Sim- on 60% of length - - Cluster# 0
# seqs 93
Clustered at 45%-Sim- on 60% of length - - Cluster# 0
# seqs 0
Clustered at 55%-Sim- on 60% of length - - Cluster# 0
# seqs 0
Clustered at 65%-Sim- on 60% of length - - Cluster# 0
# seqs 0
Clustered at 75%-Sim- on 6% of length - - Cluster# 1719
# seqs 1
Clustered at 85%-Sim- on 60% of length - - Cluster# 1564
# seqs 1
Clustered at 95%-Sim- on 60% of length - - Cluster# 1417
# seqs 1



User Contributed Notes
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AY062189.1_s_at


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