anopheles gambiae gene expression profile @uci

Probeset ID	AY062190.1_s_at
Current gene ID	AGAP001864 (AGAP001864 is also associated with: Ag.2R.4260.0_at)
Affymetrix transcript data	AY062190.1 /DEF=Anopheles gambiae cytochrome P450 CYP4H15 (Cyp4H15) mRNA, partial cds. /DB_XREF=gi:18139568

STAGE	SIGNAL MEAN	STD DEV
L	186.23	26.98
M	101.53	87.27
NBF	241.5	2.21
BF3h	1176.37	170.22
BF24h	460.93	8.88
BF48h	138.3	3.55
BF72h	136.83	3.4
BF96h	212.5	10.92
BF15d	212.57	19.47

TISSUE	SIGNAL MEAN	STD DEV
Fat bodies	396.87	85.36
Midgut	6459.77	445.02
Ovaries	335.57	39.99

ENSEMBL ANNOTATION
VECTORBASE ANNOTATION
USER-CONTRIBUTED NOTES (47)

AFFYMETRIX ANNOTATION
Probeset ID	AY062190.1_s_at
Annotation Date	3/8/2007
Genome Version	February 2003 (IAGP v.MOZ2)
Alignments	chr2R:11615181-11615605 (+) // 92.27 //
Ensembl	ENSANGG00000020674
SwissProt	Q8WQP7
Gene Ontology Biological Process	0006118 // electron transport // inferred from electronic annotation
Gene Ontology Molecular Function	0004497 // monooxygenase activity // inferred from electronic annotation /// 0005506 // iron ion binding // inferred from electronic annotation /// 0016491 // oxidoreductase activity // inferred from electronic annotation /// 0020037 // heme binding // in
InterPro	IPR001128 // Cytochrome P450 // 2.8E-57 /// IPR001128 // Cytochrome P450 // 3.0E-58
Trans Membrane	SNAP_ANOPHELES00000005019 // span:4-22,35-53 // numtm:2
Annotation Description	This probe set was annotated using the Matching Probes based pipeline to a Entrez Gene identifier using 1 transcripts. // false // Matching Probes // A
Annotation Transcript Cluster	XM_321204(11)
Transcript Assignments	AY062190 // Anopheles gambiae cytochrome P450 CYP4H15 (Cyp4H15) mRNA, partial cds. // gb // 11 // --- /// SNAP_ANOPHELES00000005019 // cdna:SNAP chromosome:AgamP3:2R:11614187:11615787:1 // ensembl_prediction // 11 // --- /// ENSANGT00000024471 // cdna:kno
Annotation Notes	SNAP_ANOPHELES00000005021 // ensembl_prediction // 7 // Cross Hyb Matching Probes /// ENSANGT00000032420 // ensembl_transcript // 7 // Cross Hyb Matching Probes

ANOXCEL ANNOTATION
Seq name	AGAP001864-PA
First residue	T
Description	pep:known chromosome:AgamP3:2R:11614175:11618749:1 gene:AGAP001864 transcript:AGAP001864-RA
CDS name and link to nucleotide data	AGAP001864-RA
Description	cdna:known chromosome:AgamP3:2R:11614175:11618749:1 gene:AGAP001864
Stop?	*
Best match to AG-CDS	AGAP001864-RA
E value	0
Score	1058
Extent of match	534
Length of best match	1605
% identity	100
% Match length	33
First residue of match - UTR overhang	1
First residue of sequence	1
Link to whole gene + 2000nt 5' + 200nt 3'	AGAP001864-PA
Link to 5' 2,000 nt	AGAP001864-PA
Link to 3' 200 nt	AGAP001864-PA
Gene name and view at Ensembl	AGAP001864
Protein view at Ensembl	AGAP001864-PA
Chromosome	2R
For or Rev	F
Location	join(11614175..11614818,11614887..11615136,11618039..11618749)
Exon locations	11614175-11614818 11614887-11615136 11618039-11618749
Number of introns and link to fasta file	2
intron lengths	69 2903
Start	11614175
End	11618749
Gene length	4574
Protein size	534
Complexity index	0.25
Cys number	6
SigP Result	SIG
Cleavage Position	16-17
MW	61.58
pI	6.57
Mature MW	59.842
pI	6.44
TMHMM result	ExpAA=38.69 First60=33.94 PredHel=2 Topology=o5-27i39-57o
Predicted helices	2
% membrane	7.5
% outside	90.3
% inside	2.2
Best match to NR protein database	cytochrome P450
E value	1E-177
Match	gi\|108876205
Score	625
Extent of match	529
Length of best match	512
% identity	56
% Match length	103
First residue of match	4
First residue of sequence	7
Number of segments	1
Species	Aedes aegypti
Key words	CYTOCHROME P450 AEDES AEGYPTI SIMILAR TO CG3466-PA TRIBOLIUM CASTANEUM DROSOPHILA MELANOGASTER >GI CP4D2_DROME 4D2 CYPIVD2 SIMULANS PSEUDOOBSCURA CYP4D14 CG3540-PA C4D14_DROME PROBABLE 4D14 CYPIVD14 4C3 CYPIVC3 ISOFORM 1 P 450 FRUIT FLY 4 FAMILY DAPHNIA MAGNA CP4C1_BLADI 4C1 CYPIVC1
Best match to SWISSP database	Cytochrome P450 4d2 (EC 1.14.-.-) (CYPIVD2) - Drosophila melanogaster (Fruit
E value	9E-098
Match	Q27589\|CP4D2_DROME
Score	357
Extent of match	491
Length of best match	501
% identity	41
% Match length	98
First residue of match	37
First residue of sequence	48
Number of segments	1
PROSITE motifs	ASN_GLYCOSYLATION \|CAMP_PHOSPHO_SITE \|PKC_PHOSPHO_SITE \|CK2_PHOSPHO_SITE \|TYR_PHOSPHO_SITE \|MYRISTYL \|AMIDATION \|PRENYLATION \|ER_TARGET \|MICROBODIES_CTER \|CYTOCHROME_P450 \|
Non promiscuous prosite motifs	ER_TARGET \|CYTOCHROME_P450 \|
Best match to GO database	Cytochrome P450-4d2 - Drosophila melanogaster - electron carrier activity - microsome - membrane
E value	E-9
Function descriptors	molecular_function
Function parent	molecular_function
GO #	GO:0003674
E value of functional GO	1e-90
Component descriptors	cellular_component
Component parent	cellular_component
GO #	GO:0005575
E value of component GO	1e-90
Process descriptors	biological_process
Process parent	molecular_function
GO #	GO:0008150
E value of process GO	1e-90
Best match to KOG database	Cytochrome P450 CYP4/CYP19/CYP26 subfamilies
E value	1E-097
General class	Secondary metabolites biosynthesis, transport and catabolism, Lipid transport and metabolism
Best match to PFAM database	p450
E value	2E-077
Best match to AEGY-PEP database	cytochrome P450
E value	1e-179
Match	AAEL007830-RA
Score	625
Extent of match	529
Length of best match	512
% identity	56
% Match length	103
First residue of match	4
First residue of sequence	7
Number of segments	1
Best match to DMPROT database	Cyp4d2-PA
E value	1E-098
Match	Cyp4d2-PA
Score	357
Extent of match	491
Length of best match	501
% identity	41
% Match length	98
First residue of match	37
First residue of sequence	48
Number of segments	1
Best match to CELEG database	cyp-31A2 Cytochrome P450 status:Partially_confirmed TR:O45605
E value	7E-077
Match	H02I12.8
Score	284
Extent of match	503
Length of best match	487
% identity	33
% Match length	103
First residue of match	25
First residue of sequence	34
Number of segments	1
Best match to ARAB database	cytochrome P450 family prote
E value	1E-033
Match	AT5G24900.1
Score	141
Extent of match	489
Length of best match	525
% identity	25
% Match length	93
First residue of match	86
First residue of sequence	65
Number of segments	1
Best match to YEAST database	SW:ERG5_YEAST P54781 saccharomyces cerevisiae (baker's yeast). cytochrome p450
E value	3E-018
Match	NR_SC:SW-ERG5_YEAST
Score	88.6
Extent of match	363
Length of best match	538
% identity	23
% Match length	67
First residue of match	172
First residue of sequence	203
Number of segments	1
Best match to clusterized An. gambiae ESTs from different libraries	all-ests-contig_6256
E value	1e-123
Score	437
Extent of match	520
Length of best match	1706
% identity	43
% Match length	30
First residue of match	95
First residue of sequence	21
Number of segments	1
Orientation of output	FOR
Number of sequences on assembled contig	8
ESTs coming from from all-instars library	2
Blood-abdomen library	0
Sugar-abd library	0
Infected blood-abd library	0
Big blood fed library	1
Big non blood fed library	5
Head library	0
Immune cells	0
normalized-fatbody	0
Female salivary glands (Ribeiro and Pasteur)	0
Male salivary glands	0
Best match to Affymetrix chip	Ag.2R.2486.0_CDS_at
E value	0
Extent of match	526
% identity	94
Clustered at 35%-Sim- on 60% of length - - Cluster#	0
# seqs	93
Clustered at 45%-Sim- on 60% of length - - Cluster#	0
# seqs	0
Clustered at 55%-Sim- on 60% of length - - Cluster#	0
# seqs	0
Clustered at 65%-Sim- on 60% of length - - Cluster#	0
# seqs	0
Clustered at 75%-Sim- on 6% of length - - Cluster#	0
# seqs	0
Clustered at 85%-Sim- on 60% of length - - Cluster#	9703
# seqs	1
Clustered at 95%-Sim- on 60% of length - - Cluster#	9904
# seqs	1