anopheles gambiae gene expression profile @uci

Probeset ID	Ag.2L.1020.2_CDS_a_at
Current gene ID	AGAP007452
Affymetrix transcript data	ENSANGT00000026269 /FEA=ENS /TIER=CDS /ANNOT=ENSANGT00000026269 /EVID_CNT=1 /EVID_TYPE=ENS /GENE_CLUSTER=AffxAg.1020 /SEG=chr2L:-46099298,46104390

STAGE	SIGNAL MEAN	STD DEV
L	154.27	18.67
M	130.97	37.95
NBF	193.23	29.94
BF3h	214.1	29.64
BF24h	267.83	38.8
BF48h	292.9	33.69
BF72h	303.57	20.27
BF96h	213.2	39.12
BF15d	227.9	17.03

TISSUE	SIGNAL MEAN	STD DEV
Fat bodies	391.1	35.76
Midgut	390.17	86.62
Ovaries	167.87	60.86

ENSEMBL ANNOTATION
VECTORBASE ANNOTATION
USER-CONTRIBUTED NOTES (47)

AFFYMETRIX ANNOTATION
Probeset ID	Ag.2L.1020.2_CDS_a_at
Annotation Date	3/8/2007
Target Description	ens:ENSANGT00000026269 /FEA=ENS /TIER=CDS /ANNOT=ens:ENSANGT00000026269 /EVID_CNT=1 /EVID_TYPE=ENS /GENE_CLUSTER=AffxAg.1020 /SEG=chr2L:-46099298,46104390
Genome Version	February 2003 (IAGP v.MOZ2)
Alignments	chr2L:46099298-46104390 (-) // 100.0 //
Annotation Description	This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcripts. // false // Matching Probes // A
Annotation Transcript Cluster	XM_564795(11)
Transcript Assignments	XM_564795 // Anopheles gambiae str. PEST ENSANGP00000027416 (ENSANGG00000022589), partial mRNA. // refseq // 11 // ---
Annotation Notes	SNAP_ANOPHELES00000005889 // ensembl_prediction // 6 // Cross Hyb Matching Probes

ANOXCEL ANNOTATION
Seq name	AGAP007452-PA
First residue	M
Description	pep:novel chromosome:AgamP3:2L:46638581:46673632:-1 gene:AGAP007452 transcript:AGAP007452-RA
CDS name and link to nucleotide data	AGAP007452-RA
Description	cdna:novel chromosome:AgamP3:2L:46638581:46673632:-1 gene:AGAP007452
ATG?	ATG
Best match to AG-CDS	AGAP007452-RA
E value	0
Score	5451
Extent of match	2737
Length of best match	8592
% identity	100
% Match length	32
First residue of match - UTR overhang	1
First residue of sequence	1
Link to whole gene + 2000nt 5' + 200nt 3'	AGAP007452-PA
Link to 5' 2,000 nt	AGAP007452-PA
Link to 3' 200 nt	AGAP007452-PA
Gene name and view at Ensembl	AGAP007452
Protein view at Ensembl	AGAP007452-PA
Chromosome	2L
For or Rev	R
Location	join(complement(46668702..46673632),complement(46668546..46668625),complement(46644977..46645470),complement(46643452..46644640),complement(46642533..46643385),complement(46642298..46642460),complement(46639329..46639533),complement(46638959..46639257))
Exon locations	46668702-46673632 46668546-46668625 46644977-46645470 46643452-46644640 46642533-46643385 46642298-46642460 46639329-46639533 46638959-46639257
Number of introns and link to fasta file	7
intron lengths	77 23076 337 67 73 2765 72
Start	46638959
End	46673632
Gene length	34673
Protein size	2737
Complexity index	0.21
Cys number	24
SigP Result	CYT
MW	298.83
pI	6.67
TMHMM result	ExpAA=0.00 First60=0.00 PredHel=0 Topology=o
Predicted helices	0
Best match to NR protein database	guanine nucleotide exchange factor
E value	0.0
Match	gi\|108883231
Score	1137
Extent of match	970
Length of best match	867
% identity	64
% Match length	112
First residue of match	1
First residue of sequence	1745
Number of segments	1
Species	Aedes aegypti
Key words	GUANINE NUCLEOTIDE EXCHANGE FACTOR AEDES AEGYPTI CG3799-PA ISOFORM A DROSOPHILA MELANOGASTER >GI CG3799-PB CG3799-PC BCDNA.GH03693 EPHEXIN PSEUDOOBSCURA SIMILAR TO TRIBOLIUM CASTANEUM HYPOTHETICAL AAEL_AAEL001436 1 APIS MELLIFERA CAENORHABDITIS BRIGGSAE TEMPORARILY ASSIGNED GENE NAME FAMILY MEMBER TAG-218 ELEGANS TAG B DANIO RERIO NOVEL VERTEBRATE RHO GEF 15 ARHGEF15 TAURUS NEURONAL Q8CHT1 NGEF_MOUSE EPHEXIN-1 EPH-INTERACTING MUSCULUS CRA_A EPH INTERACTING Q5BKC9 NGEF_RAT RATTUS NORVEGICUS EPHEXIN1 GALLUS MACACA MULATTA Q5RDX5 NGEF_PONPY PONGO PYGMAEUS MUS SYNTHETIC CONSTRUCT CRA_C HOMO SAPIENS UNKNOWN
Best match to SWISSP database	Ephexin-1 (Eph-interacting exchange protein) (Neuronal guanine nucleotide
E value	2E-075
Match	Q8CHT1\|NGEF_MOUSE
Score	285
Extent of match	629
Length of best match	710
% identity	32
% Match length	89
First residue of match	93
First residue of sequence	2050
Number of segments	1
PROSITE motifs	ASN_GLYCOSYLATION \|CAMP_PHOSPHO_SITE \|PKC_PHOSPHO_SITE \|CK2_PHOSPHO_SITE \|TYR_PHOSPHO_SITE \|MYRISTYL \|AMIDATION \|PRENYLATION \|ER_TARGET \|MICROBODIES_CTER \|RGD \|
Non promiscuous prosite motifs	ER_TARGET \|RGD \|
Best match to GO database	ARHGEF5, TIM: Rho guanine nucleotide exchange factor 5 - Homo sapiens - protein binding - GTP binding
E value	E-6
Function descriptors	molecular_function
Function parent	molecular_function
GO #	GO:0003674
E value of functional GO	5e-62
Component descriptors	cellular_component
Component parent	cellular_component
GO #	GO:0005575
E value of component GO	5e-62
Process descriptors	biological_process
Process parent	molecular_function
GO #	GO:0008150
E value of process GO	5e-62
Best match to KOG database	Putative guanine nucleotide exchange factor TIM
E value	1E-141
General class	Signal transduction mechanisms
Best match to PFAM database	RhoGEF
E value	5E-013
Best match to SMART database	RhoGEF
E value	1E-031
Best match to AEGY-PEP database	guanine nucleotide exchange factor
E value	0
Match	AAEL001398-RA
Score	1137
Extent of match	970
Length of best match	867
% identity	64
% Match length	112
First residue of match	1
First residue of sequence	1745
Number of segments	1
Best match to DMPROT database	CG3799-PB
E value	0.0
Match	CG3799-PB
Score	799
Extent of match	1066
Length of best match	1051
% identity	45
% Match length	101
First residue of match	118
First residue of sequence	1671
Number of segments	1
Best match to CELEG database	Partially_confirmed TR:O76572 protein_id:AAC26284.1 Length = 648 Score = 339
E value	1E-092
Match	K07D4.7b
Score	339
Extent of match	524
Length of best match	648
% identity	38
% Match length	81
First residue of match	105
First residue of sequence	2176
Number of segments	1
Best match to ARAB database	expressed protein
E value	4E-013
Match	gi\|15228477
Score	75.5
Extent of match	895
Length of best match	2081
% identity	18
% Match length	43
First residue of match	187
First residue of sequence	470
Number of segments	4
Best match to YEAST database	gi\|677198\|gb\|AAB00143.1 (L03188) putative
E value	1E-010
Match	NR_SC:GP-AAB00143_1
Score	66.2
Extent of match	876
Length of best match	1015
% identity	19
% Match length	86
First residue of match	85
First residue of sequence	567
Number of segments	2
Best match to clusterized An. gambiae ESTs from different libraries	all-ests-contig_6281
E value	1e-170
Score	596
Extent of match	281
Length of best match	1224
% identity	100
% Match length	23
First residue of match	1
First residue of sequence	2457
Number of segments	1
Orientation of output	FOR
Satisfied 95% id on length 100	Y
Number of sequences on assembled contig	8
ESTs coming from from all-instars library	1
Blood-abdomen library	0
Sugar-abd library	0
Infected blood-abd library	0
Big blood fed library	0
Big non blood fed library	0
Head library	7
Immune cells	0
normalized-fatbody	0
Female salivary glands (Ribeiro and Pasteur)	0
Male salivary glands	0
Best match to Affymetrix chip	Ag.2L.1020.2_CDS_a_at
E value	0
Extent of match	815
% identity	95
Clustered at 35%-Sim- on 60% of length - - Cluster#	4690
# seqs	1
Clustered at 45%-Sim- on 60% of length - - Cluster#	5323
# seqs	1
Clustered at 55%-Sim- on 60% of length - - Cluster#	5730
# seqs	1
Clustered at 65%-Sim- on 60% of length - - Cluster#	6063
# seqs	1
Clustered at 75%-Sim- on 6% of length - - Cluster#	6233
# seqs	1
Clustered at 85%-Sim- on 60% of length - - Cluster#	6367
# seqs	1
Clustered at 95%-Sim- on 60% of length - - Cluster#	6490
# seqs	1