anopheles gambiae gene expression profile @uci

Probeset ID	Ag.2L.103.0_CDS_at
Current gene ID	AGAP006716
Affymetrix transcript data	ENSANGT00000024062 /FEA=ENS /TIER=CDS /GEN=GPRgr27 /ANNOT=ENSANGT00000024062 /EVID_CNT=1 /EVID_TYPE=ENS /GENE_CLUSTER=AffxAg.103 /SEG=chr2L:+36579437,36580821

STAGE	SIGNAL MEAN	STD DEV
L	89.63	14.97
M	99.83	59.95
NBF	107.1	57.16
BF3h	219.87	34.99
BF24h	106.03	33.6
BF48h	134.67	45.05
BF72h	96.77	16.53
BF96h	94.23	4.91
BF15d	93.5	41.05

TISSUE	SIGNAL MEAN	STD DEV
Fat bodies	97.13	49.7
Midgut	106.57	21.59
Ovaries	110.53	6.57

ENSEMBL ANNOTATION
VECTORBASE ANNOTATION
USER-CONTRIBUTED NOTES (47)

AFFYMETRIX ANNOTATION
Probeset ID	Ag.2L.103.0_CDS_at
Annotation Date	3/8/2007
Target Description	ens:ENSANGT00000024062 /FEA=ENS /TIER=CDS /GEN=GPRgr27 /ANNOT=ens:ENSANGT00000024062 /EVID_CNT=1 /EVID_TYPE=ENS /GENE_CLUSTER=AffxAg.103 /SEG=chr2L:+36579437,36580821
Genome Version	February 2003 (IAGP v.MOZ2)
Alignments	chr2L:36579437-36580821 (+) // 100.0 //
Ensembl	ENSANGG00000019942
Trans Membrane	ENSANGESTP00000012512 // span:55-77,87-109,185-207,257-279,374-396 // numtm:5 /// ENSANGP00000022361 // span:55-77,87-109,185-207,257-279,374-396 // numtm:5
Annotation Description	This probe set was annotated using the Matching Probes based pipeline to a Entrez Gene identifier using 1 transcripts. // false // Matching Probes // A
Annotation Transcript Cluster	XM_309027(11)
Transcript Assignments	ENSANGESTT00000012512 // --- // ensembl_est // 11 // --- /// ENSANGT00000024062 // cdna:known chromosome:AgamP3:2L:37148677:37150060:1 gene:ENSANGG00000019942 // ensembl_transcript // 11 // --- /// XM_309027 // Anopheles gambiae str. PEST ENSANGP000000223
Annotation Notes	SNAP_ANOPHELES00000004089 // ensembl_prediction // 7 // Cross Hyb Matching Probes

ANOXCEL ANNOTATION
Seq name	AGAP006716-PA
First residue	M
Description	pep:known chromosome:AgamP3:2L:37148677:37150060:1 gene:AGAP006716 transcript:AGAP006716-RA
CDS name and link to nucleotide data	AGAP006716-RA
Description	cdna:known chromosome:AgamP3:2L:37148677:37150060:1 gene:AGAP006716
ATG?	ATG
Stop?	*
Best match to AG-CDS	AGAP006716-RA
E value	0
Score	816
Extent of match	407
Length of best match	1224
% identity	100
% Match length	33
First residue of match - UTR overhang	1
First residue of sequence	1
Link to whole gene + 2000nt 5' + 200nt 3'	AGAP006716-PA
Link to 5' 2,000 nt	AGAP006716-PA
Link to 3' 200 nt	AGAP006716-PA
Gene name and view at Ensembl	AGAP006716
Protein view at Ensembl	AGAP006716-PA
Chromosome	2L
For or Rev	F
Location	join(37148677..37149726,37149796..37149894,37149986..37150060)
Exon locations	37148677-37149726 37149796-37149894 37149986-37150060
Number of introns and link to fasta file	2
intron lengths	70 92
Start	37148677
End	37150060
Gene length	1383
Protein size	407
Complexity index	0.31
Cys number	12
SigP Result	CYT
MW	45.769
pI	9.23
TMHMM result	ExpAA=139.08 First60=6.24 PredHel=5 Topology=i55-77o87-109i185-207o257-279i374-396o
Predicted helices	5
% membrane	27
% outside	17.4
% inside	55.5
Best match to NR protein database	Gustatory receptor 59f CG33150-PA [Drosophila melanogaster]
E value	6E-009
Match	gi\|28573623
Score	65.5
Extent of match	316
Length of best match	406
% identity	22
% Match length	78
First residue of match	98
First residue of sequence	91
Number of segments	1
Species	Drosophila melanogaster
Key words	GUSTATORY RECEPTOR 59F DROSOPHILA MELANOGASTER >GI GR59F_DROME PSEUDOOBSCURA SIMILAR TO ISOFORM B TRIBOLIUM CASTANEUM CANDIDATE 55 43A CG1712-PA MELLIFERA CHEMOSENSORY 4 HELIOTHIS VIRESCENS CG1712-PB A GR43A_DROME CONSERVED HYPOTHETICAL AEDES AEGYPTI 28B E 59E GR59E_DROME 66A D C GR28B_DROME BACN32G11.1 2A GR02A_DROME OLFACTORY BOMBYX MORI
Best match to SWISSP database	Putative gustatory receptor 59f - Drosophila melanogaster (Fruit fly)
E value	4E-010
Match	Q9W1N5\|GR59F_DROME
Score	65.5
Extent of match	316
Length of best match	406
% identity	22
% Match length	78
First residue of match	98
First residue of sequence	91
Number of segments	1
PROSITE motifs	PKC_PHOSPHO_SITE \|CK2_PHOSPHO_SITE \|MYRISTYL \|AMIDATION \|PRENYLATION \|MICROBODIES_CTER \|
Best match to GO database	Gustatory receptor 59f - Drosophila melanogaster - sensory perception of taste - taste receptor activity - integral to membrane
E value	.000000000
Function descriptors	taste receptor activity\|\|rhodopsin-like receptor activity\|\|G-protein coupled receptor activity\|\|transmembrane receptor activity\|\|receptor activity\|\|signal transducer activity
Function parent	signal transducer activity
Function second parent	receptor activity
GO #	GO:0008527
E value of functional GO	1e-10
Component descriptors	integral to membrane\|\|intrinsic to membrane\|\|endomembrane system\|\|membrane
Component parent	membrane
Component second parent	endomembrane system
GO #	GO:0016021
E value of component GO	1e-10
Process descriptors	sensory perception of taste\|\|sensory perception of chemical stimulus\|\|sensory perception\|\|neurophysiological process\|\|organismal physiological process
Process parent	signal transducer activity
Process second parent	receptor activity
GO #	GO:0050909
E value of process GO	1e-10
Best match to KOG database	Uncharacterized conserved protein
E value	0.46
General class	Function unknown
Best match to PFAM database	7tm_7
E value	4E-034
Best match to SMART database	FYVE
E value	0.15
Best match to AEGY-PEP database	conserved hypothetical protein
E value	5e-07
Match	AAEL013196-RA
Score	51.6
Extent of match	337
Length of best match	398
% identity	23
% Match length	85
First residue of match	69
First residue of sequence	93
Number of segments	1
Best match to DMPROT database	Gr59f-PA
E value	5E-011
Match	Gr59f-PA
Score	65.5
Extent of match	316
Length of best match	406
% identity	22
% Match length	78
First residue of match	98
First residue of sequence	91
Number of segments	1
Best match to CELEG database	gur-2 chemoreceptor status:Partially_confirmed TR:Q17990 protein_id:AAA80362.3
E value	0.58
Match	C14F11.3
Score	32
Extent of match	230
Length of best match	439
% identity	17
% Match length	52
First residue of match	210
First residue of sequence	176
Number of segments	1
Best match to ARAB database	expressed protein \| chr3:20456389-20463377 REVERSE \| Aliases: T26I12.40 Length =
E value	2.6
Match	AT3G55160.1
Score	30
Extent of match	95
Length of best match	2149
% identity	27
% Match length	4
First residue of match	327
First residue of sequence	95
Number of segments	1
Best match to YEAST database	SW:TF3B_YEAST P29056 saccharomyces cerevisiae (baker's yeast). transcription
E value	8.2
Match	NR_SC:SW-TF3B_YEAST
Score	26.9
Extent of match	30
Length of best match	596
% identity	33
% Match length	5
First residue of match	25
First residue of sequence	368
Number of segments	1
Best match to clusterized An. gambiae ESTs from different libraries	all-ests-contig_17376
E value	2e-09
Score	59.7
Extent of match	206
Length of best match	679
% identity	27
% Match length	30
First residue of match	3
First residue of sequence	198
Number of segments	1
Orientation of output	FOR
Number of sequences on assembled contig	1
ESTs coming from from all-instars library	1
Blood-abdomen library	0
Sugar-abd library	0
Infected blood-abd library	0
Big blood fed library	0
Big non blood fed library	0
Head library	0
Immune cells	0
normalized-fatbody	0
Female salivary glands (Ribeiro and Pasteur)	0
Male salivary glands	0
Best match to Affymetrix chip	Ag.2L.103.0_CDS_at
E value	0
Extent of match	407
% identity	99
Clustered at 35%-Sim- on 60% of length - - Cluster#	0
# seqs	164
Clustered at 45%-Sim- on 60% of length - - Cluster#	0
# seqs	0
Clustered at 55%-Sim- on 60% of length - - Cluster#	5228
# seqs	1
Clustered at 65%-Sim- on 60% of length - - Cluster#	5484
# seqs	1
Clustered at 75%-Sim- on 6% of length - - Cluster#	5625
# seqs	1
Clustered at 85%-Sim- on 60% of length - - Cluster#	5712
# seqs	1
Clustered at 95%-Sim- on 60% of length - - Cluster#	5764
# seqs	1